Mating of 2 laboratory saccharomyces cerevisiae strains resulted in enhanced production of 2-phenylethanol by biotransformation of l-phenylalanine
PBN-AR
Instytucja
Wydział Chemiczny (Politechnika Warszawska)
Informacje podstawowe
Główny język publikacji
en
Czasopismo
Journal of Molecular Microbiology and Biotechnology (25pkt w roku publikacji)
ISSN
1464-1801
EISSN
Wydawca
S. Karger AG
DOI
Rok publikacji
2017
Numer zeszytu
2
Strony od-do
81-90
Numer tomu
27
Identyfikator DOI
Liczba arkuszy
0.5
Autorzy
Pozostali autorzy
+ 1
Słowa kluczowe
en
2-Phenylethanol · Yeast · Diploid · Biotransformation · Biotechnology
Streszczenia
Język
en
Treść
2-Phenylethanol (2-PE) is an aromatic alcohol with a rosy scent which is widely used in the food, fragrance, and cosmetic industries. Promising sources of natural 2-PE are microorganisms, especially yeasts, which can produce 2-PE by biosynthesis and biotransformation. Thus, the first challenging goal in the development of biotechnological production of 2-PE is searching for highly productive yeast strains. In the present work, 5 laboratory Saccharomyces cerevisiae strains were tested for the production of 2-PE. Thereafter, 2 of them were hybridized by a mating procedure and, as a result, a new diploid, S. cerevisia AM1-d, was selected. Within the 72-h batch culture in a medium containing 5 g/L of L-phenyl-alanine, AM1-d produced 3.83 g/L of 2-PE in a shaking flask. In this way, we managed to select the diploid S. cerevisiae AM1-d strain, showing a 3- and 5-fold increase in 2-PE production in comparison to parental strains. Remarkably, the enhanced production of 2-PE by the hybrid of 2 yeast laboratory strains is demonstrated here for the first time.
Inne
System-identifier
WUT2e34cc2f54ce4f1b9c37e3172df762e5
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