Bioaccumulation, Antioxidative Response, and Metallothionein Expression in Lupinus luteus L. Exposed to Heavy Metals and Silver Nanoparticles
PBN-AR
Instytucja
Wydział Infrastruktury i Środowiska (Politechnika Częstochowska)
Informacje podstawowe
Główny język publikacji
en
Czasopismo
Environmental Science and Pollution Research
ISSN
0944-1344
EISSN
Wydawca
Springer
URL
Rok publikacji
2019
Numer zeszytu
Iss.16
Strony od-do
16040-16052
Numer tomu
Vol.26
Identyfikator DOI
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Autorzy
Pozostali autorzy
+ 3
Słowa kluczowe
en
heavy metals
phytoremediation
Bioremediation
biomarkers
phytotoxicity
oxidative stress
Streszczenia
Język
en
Treść
Yellow-lupin (Lupinus luteus L.) was grown on soils contaminated with heavy metals during two parallel studies. In the first one, the soil was contaminated by industrial activities whereas, in the second one, the soil was artificially contaminated with a single metal including Cd, Pb, Zn, Ni (in nitrate form), and Ag (in nitrate and nanoparticles form). The study was performed to assess a plant’s response to contamination including its antioxidative response and molecular mechanisms involved in metal detoxification through the expression level of metallothioneins (MTs). Overall, the study provided insights into identification and validation of housekeeping genes (HKG) in L. luteus under exposure to metal stress and showed the effects of selected heavy metals and silver nanoparticles on the expression of metallothioneins, the activity of guaiacol peroxidase (GPX) and bioaccumulation of metals in leaves of L. luteus. As such, HKG validation using BestKeeper, NormFinder, and geNorm software allowed for the selection of four most stable reference genes in a context metal contamination for the selected plant. Moreover, a significant increase in the expression levels of MT was observed in plants grown under heavy metal stress and none on plants grown on 25 mg kg−1 of silver nanoparticles. Also, the GPX activity and MT expression showed statistically significant changes between different conditions and doses which means that they can be used as highly sensitive stress markers for planning the phytoremediation process on a large scale.
Cechy publikacji
original-article
Inne
System-identifier
61323
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