Scaffolds for Chondrogenic Cells Cultivation Prepared from Bacterial Cellulose with Relaxed Fibers Structure Induced Genetically.
PBN-AR
Instytucja
Wydział Biotechnologii i Nauk o Żywności (Politechnika Łódzka)
Informacje podstawowe
Główny język publikacji
en
Czasopismo
Nanomaterials (35pkt w roku publikacji)
ISSN
2079-4991
EISSN
Wydawca
MDPI - Open Access Publishing
DOI
Rok publikacji
2018
Numer zeszytu
12
Strony od-do
1066
Numer tomu
8
Identyfikator DOI
Liczba arkuszy
Słowa kluczowe
en
bacterial nano-cellulose
3D culture
scaffold
chondrogenic differentiation
Komagataeibacter
bacterial cellulose fiber structure
Streszczenia
Język
en
Treść
Development of three-dimensional scaffolds mimicking in vivo cells’ environment is an ongoing challenge for tissue engineering. Bacterial nano-cellulose (BNC) is a well-known biocompatible material with enormous water-holding capacity. However, a tight spatial organization of cellulose fibers limits cell ingrowth and restricts practical use of BNC-based scaffolds. The aim of this study was to address this issue avoiding any chemical treatment of natural nanomaterial. Genetic modifications of Komagataeibacter hansenii ATCC 23769 strain along with structural and mechanical properties characterization of obtained BNC membranes were conducted. Furthermore, the membranes were evaluated as scaffolds in in vitro assays to verify cells viability and glycosaminoglycan synthesis by chondrogenic ATDC5 cells line as well as RBL-2H3 mast cells degranulation. K. hansenii mutants with increased cell lengths and motility were shown to produce BNC membranes with increased pore sizes. Novel, BNC membranes with relaxed fiber structure revealed superior properties as scaffolds when compared to membranes produced by a wild-type strain. Obtained results confirm that a genetic modification of productive bacterial strain is a plausible way of adjustment of bacterial cellulose properties for tissue engineering applications without the employment of any chemical modifications.
Cechy publikacji
discipline:Biotechnologia – dziedzina nauk technicznych
discipline:Biotechnology – field of technical sciences
Original article
Original article presents the results of original research or experiment.
Oryginalny artykuł naukowy
Oryginalny artykuł naukowy przedstawia rezultaty oryginalnych badań naukowych lub eksperymentu.
Inne
System-identifier
PBN-R:906344
CrossrefMetadata from Crossref logo
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