The long-term immunological effects of alloferon and its analogues in the mealworm Tenebrio molitor.
PBN-AR
Instytucja
Wydział Chemii (Uniwersytet Wrocławski)
Informacje podstawowe
Główny język publikacji
en
Czasopismo
Insect Science (35pkt w roku publikacji)
ISSN
1672-9609
EISSN
1744-7917
Wydawca
John Wiley and Sons
Rok publikacji
2018
Numer zeszytu
Strony od-do
429-438
Numer tomu
25
Identyfikator DOI
Liczba arkuszy
Autorzy
(liczba autorów: 4)
Pozostali autorzy
+ 3
Słowa kluczowe
en
alloferon
analogue
hemocyte’s apoptosi
immune response
nodulation
phenoloxidase activity
Streszczenia
Język
en
Treść
The subject of this article is a search for the long-term immunological effects of alloferon and 3 structural analogues of alloferon, which were earlier characterized by the highest pro-apoptotic activity in Tenebrio molitor. The differences in the actions of these peptides on immune response were observed. Alloferon increased nodulation and significantly phenoloxidase activity in the hemolymph of experimentally infected T. molitor. However, [Phe(p-NH2)1]- and [Phe(p-OMe)1]-alloferon strongly inhibited cellular and humoral defense of the mealworm against Staphylococcus aureus infection. One day after injection of these peptides, the specific biochemical and morphological hallmarks of apoptosis in bacteria-challenged hemocytes were visible; in contrast, 3 days after peptides injection in all hemocytes, caspase activation was not observed. However, these new, circulating hemocytes differed from the control and the peptide-untreated bacteria-challenged hemocytes. They had an increased adhesion that led to a separation of viable, anucleated fragments of hemocytes that retain the ability to adhere and to form long filopodia. The peptide-induced separation of hemocyte fragments may resemble the formation of platelets in mammals and perhaps play a role in sealing wounds in insects. The results of in vivo studies may suggest a long half-life of studied peptides in the hemolymph of mealworm. Moreover, we showed the importance of the N-terminal histidine residues at position one of the alloferon molecule for its immunological properties in insects. The results obtained here show that alloferon plays pleiotropic functions in insects.
Cechy publikacji
original-article
Inne
System-identifier
2017CH8171
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