Comparing dried and liquid blood serum samples of depressed patients: An analysis by Raman and infrared spectroscopy methods
PBN-AR
Instytucja
Instytut Fizyki Jądrowej im. Henryka Niewodniczańskiego Polskiej Akademii Nauk
Informacje podstawowe
Główny język publikacji
en
Czasopismo
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS (35pkt w roku publikacji)
ISSN
0731-7085
EISSN
Wydawca
DOI
URL
Rok publikacji
2018
Numer zeszytu
Strony od-do
80-86
Numer tomu
150
Identyfikator DOI
Liczba arkuszy
Słowa kluczowe
en
Blood serum
Raman spectroscopy
FTIR
Depression
Streszczenia
Język
en
Treść
Depression is a serious mental illness. To study the mechanism of depression and search for new, more effective therapies, animal models are often used. Unfortunately, none of the available models reflects all the symptoms of depression. Therefore researchers are looking for new tools to diagnose depression. Unfortunately, the nowadays-available depression diagnosis methods are only psychological tests. However, it is known, that the amount of phospholipids, proteins and lipids decreases during depression. Raman and FTIR (Fourier Transform Infra Red) spectroscopies provide information on the chemical compounds in the measured sample e.g. blood serum. These spectroscopic techniques may thus become reliable and accurate tools for evaluating changes in the amount of phospholipids and proteins in depression disease. In this study differences between dried and liquid blood serum samples of healthy and depressed individuals measured by Raman (range 0–3000 cm−1) and FTIR (Fourier Transform Infrared) (range 900–3000 cm−1) spectroscopy were evaluated. The resulting spectra and accurate analysis led to the conclusion that an appropriate measurement of the background and the elimination of peaks from water had the greatest impact on the reliability of the results. Furthermore, after detailed studies of FTIR and Raman spectra of dried and liquid blood serum samples, including a complete analysis of peaks after Kramers-Kröning (KK) transformation, it was found that the sample preparation did not affect the results obtained by Raman spectroscopy. In FTIR measurements only a minimal effect on peak intensity was observed.
Cechy publikacji
peer-reviewed
original-article
Inne
System-identifier
809/2018
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