Fourier Transform Infrared (FTIR) spectroscopy of paraffin and deparafinnized bone tissue samples as a diagnostic tool for Ewing sarcoma of bones
PBN-AR
Instytucja
Instytut Fizyki Jądrowej im. Henryka Niewodniczańskiego Polskiej Akademii Nauk
Informacje podstawowe
Główny język publikacji
en
Czasopismo
INFRARED PHYSICS & TECHNOLOGY (25pkt w roku publikacji)
ISSN
1350-4495
EISSN
Wydawca
DOI
URL
Rok publikacji
2017
Numer zeszytu
Strony od-do
364-371
Numer tomu
85
Identyfikator DOI
Liczba arkuszy
Autorzy
(liczba autorów: 8)
Pozostali autorzy
+ 7
Słowa kluczowe
en
FTIR
Kramers–Kronig transformation
Ewing sarcoma
Fourier chemotherapy
Bone cancer
PCA-LDA
Streszczenia
Język
en
Treść
Introduction Ewing sarcoma (ES) is one of the most frequent malignant tumor of bones in children and adolescents. The final results of applied treatments stay still unsatisfactory. This is why introducing of new diagnostic tools like Fourier Transform Infrared (FTIR) spectroscopy into ES research is so important. Purpose The aim of this study was to establish the possibility of FTIR spectra detection from ES bone samples without preceding deparaffinization, and thus extension of Depciuch et al. research studies (2016) of bones with Ewing sarcoma. Material and methods A total 20 specimen of bones obtained from 10 patients with Ewing sarcoma were analyzed. A half of them was sampled before start of treatment as diagnostic biopsy specimen and rest from the resected tumor after neoadjuvant chemotherapy completion. FTIR spectroscopy (range 1100–3500 cm−1) (Vertex 70 Bruker spectrometer) was used for the specimen analysis. Every specimen was examined before and after deparaffinization procedure in triplicate. Results The presence of wax does not affect the surface area, absorbance, half-width or peak position observed in the spectrum peaks in the every analyzed sample. However, in the case of peak at 1234 cm−1 in deparaffinized cancerous tissues the statistically significant differences in the value of absorbance, surface area and half-width (FWHM) compared to paraffin cancerous tissues were observed but without significant impact on the final interpretation. The analysis function of the dielectric imaginary Ɛi (ω) confirm that the Lorentz function parameters for the individual peaks are the same so they do not depend from the sample preparation. Conclusions The spectra obtained from paraffin and deparaffinized bone tissues samples do not differ significantly. Paraffin dewaxing procedure is not necessary before bone specimen FTIR spectroscopy assessment what reduces the risk of tissue damage and adulterated spectrum, and gives some economical advantages.
Cechy publikacji
peer-reviewed
original-article
Inne
System-identifier
431
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