The protective effects of silybin on the cytotoxicity of thiram in human, rat and chicken cell cultures
PBN-AR
Instytucja
Państwowy Instytut Weterynaryjny - Państwowy Instytut Badawczy
Informacje podstawowe
Główny język publikacji
en
Czasopismo
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY (30pkt w roku publikacji)
ISSN
0048-3575
EISSN
1095-9939
Wydawca
ACADEMIC PRESS INC ELSEVIER SCIENCE, USA
Rok publikacji
2017
Numer zeszytu
Strony od-do
154-160
Numer tomu
143
Identyfikator DOI
Liczba arkuszy
Autorzy
(liczba autorów: 3)
Pozostali autorzy
+ 1
Słowa kluczowe
en
Thiram
Cytotoxicity
Silybin
Cytoprotection
Interaction
Cell lines
Streszczenia
Język
en
Treść
The study objective was a determination of thiram cytotoxicity and silybin cytoprotective activity in course of the fungicide impact on cell metabolism and membrane integrity. Firstly, human, rat, chicken hepatoma cells and rat myoblasts cultures were incubated with thiram. The results showed higher sensitivity of myoblasts on thiram exposure than the hepatoma cells. Among hepatoma cells, the chicken cultures were the most sensitive on the fungicide endangering. The mitochondrial activity was the most thiram affected function within all types the cell lines used. When silybin co-acted with thiram, an increase of the cell viability was recorded. The EC50-values were higher for thiram subjected to interaction with silybin than the effect of alone thiram action. The interaction mode between the studied compounds shown by combination index (CI) represented an antagonistic or an additive nature and was depended on thiram concentration, type of the cells and the assay used. Moreover, the morphology changes were dependent on silybin presence in the cell cultures subjected to thiram impact at the same time. Staining with Hoechst 33342 and propidium ioidium revealed the apoptosis cell death in the incubation cultures. Definitely, the results have shown a potential of silybin to protect the cultured cells in course of cytotoxicity induced by thiram. However, future studies taking into account other endpoints of thiram cytotoxicity pathways including species differences and the cytoprotection efficacy could be of interest.
Cechy publikacji
praca doświadczalna
Inne
System-identifier
1404
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