Fast and easy liquid chromatography–mass spectrometry method for evaluation of postharvest fruit safety by determination of mycotoxins: Fumitremorgin C and verruculogen
PBN-AR
Instytucja
Instytut Agrofizyki im. Bohdana Dobrzańskiego Polskiej Akademii Nauk
Informacje podstawowe
Główny język publikacji
angielski
Czasopismo
POSTHARVEST BIOLOGY AND TECHNOLOGY (40pkt w roku publikacji)
ISSN
0925-5214
EISSN
Wydawca
ELSEVIER SCIENCE BV
DOI
URL
Rok publikacji
2017
Numer zeszytu
131
Strony od-do
46–54
Numer tomu
Liczba arkuszy
Słowa kluczowe
angielski
Heat resistant fungi
Mycotoxin
Fruit contamination
Neosartorya
Liquid chromatography tandem mass spectrometry method
Streszczenia
Język
angielski
Treść
Fumitremorgin C and verruculogen are mycotoxins produced by heat-resistant Neosartorya fischeri. Easy and fast monitoring of their production by selective and sensitive analytical methods is crucial for ensuring postharvest quality of fruit, food safety, and thus human health and well-being. A six-minute liquid chromatography tandem mass spectrometry method (LC/MS/MS) was developed for quantification of fumitremorgin C and verruculogen in potato dextrose broth (PDB), strawberry juice, strawberry fruit, and soil. Mycotoxins were extracted from all media with the QuEChERS (Quick Easy Cheap Effective Rugged Safe) method. The lower limit of quantification (LLOQ) of the method for both mycotoxins was 1 μg L−1 for PDB and strawberry juice and 1 μg kg−1 for strawberry fruit and soil. Collision-induced dissociation of protonated fumitremorgin C and verruculogen ions was also examined by high-resolution mass spectrometry to assist researchers in non-targeted screening for mycotoxins and in identification of these two toxins. The developed LC/MS/MS method was applied to examine mycotoxin production by 45 environmental Neosartorya strains: 5 well-known strains, included in the study as reference strains, and 40 strains that were newly isolated and identified by us.
Cechy publikacji
original article
Inne
System-identifier
PX-59352133d5de675db4da03de
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