Exercise-induced heart mitochondrial cholesterol depletion influences the inhibition of mitochondrial swelling.
PBN-AR
Instytucja
Wydział Rehabilitacji i Kinezjologii (Akademia Wychowania Fizycznego i Sportu im. Jędrzeja Śniadeckiego w Gdańsku)
Informacje podstawowe
Główny język publikacji
en
Czasopismo
Experimental Physiology
ISSN
0958-0670
EISSN
1469-445X
Wydawca
WILEY-BLACKWELL
DOI
Rok publikacji
2013
Numer zeszytu
10
Strony od-do
1457-1468
Numer tomu
98
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Pozostali autorzy
+ 8
Słowa kluczowe
en
Membrane fluidity
Membranes cholesterol
Mitochondria associated
Mitochondrial raft-like microdomain
pl
Mitochondria
Cholesterol
Ciekłość błon
Błony
Open access
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Język
angielski
Treść
The significance of the reduction of the cholesterol pool in heart mitochondria after exercise is still unknown. Recently, published data have suggested that cholesterol may influence the components of mitochondrial contact site and affect mitochondrial swelling. Therefore, the aim of this study was to determine whether the decreased cholesterol content in heart mitochondria caused by prolonged swimming may provoke changes in their bioenergetics and result in an increased resistance to calcium chloride-induced mitochondrial swelling. Male Wistar rats were divided into a sedentary control group and an exercise group. The rats exercised for 3 h, burdened with an additional 3% of their body weight. Their hearts were removed immediately after completing the exercise. The left ventricle was divided and used for experiments. Mitochondrial cholesterol content, membrane fluidity and mitochondrial bioenergetics were measured in the control and exercised rat heart mitochondria. To assess whether mitochondrial modifications are linked to disruption of lipid microdomains, methyl-β-cyclodextrin, a well-known lipid microdomain-disrupting agent and cholesterol chelator, was applied to the mitochondria of the control group. Cholesterol depletion, increased membrane fluidity and increased resistance to calcium chloride-induced swelling were observed in postexercise heart crude mitochondrial fraction. Similar results were achieved in control mitochondria treated with 2% methyl-β-cyclodextrin. All of the mitochondrial bioenergetics parameters were similar between the groups. Therefore, the disruption of raft-like microdomains appears to be an adaptive change in the rat heart following exercise.
Cechy publikacji
ORIGINAL_ARTICLE
Inne
System-identifier
EX-585890bd82cef6c3bad1e994
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