An avian influenza H5N1 virus vaccine candidate based on the extracellular domain produced in yeast system as subviral particles protects chickens from lethal challenge
PBN-AR
Instytucja
Państwowy Instytut Weterynaryjny - Państwowy Instytut Badawczy
Informacje podstawowe
Główny język publikacji
en
Czasopismo
ANTIVIRAL RESEARCH
ISSN
0166-3542
EISSN
1872-9096
Wydawca
ELSEVIER SCIENCE BV, NETHERLANDS
Rok publikacji
2016
Numer zeszytu
Strony od-do
242-249
Numer tomu
133
Liczba arkuszy
Autorzy
Pozostali autorzy
+ 6
Słowa kluczowe
en
Avian influenza
H5N1
Recombinant hemagglutinin
Pichia pastoris
Streszczenia
Język
en
Treść
Highly pathogenic avian influenza is an on-going problem in poultry and a potential human pandemic threat. Pandemics occur suddenly and vaccine production must be fast and effective to be of value in controlling the spread of the virus. In this study we evaluated the potential of a recombinant protein from the extracellular domain of an H5 hemagglutinin protein produced in a yeast expression system to act as an effective vaccine. Protein production was efficient, with up to 200 mg purified from 1 L of culture medium. We showed that the deletion of the multibasic cleavage site from the protein improves oligomerization and, consequentially, its immunogenicity. We also showed that immunization with this deleted protein protected chickens from challenge with a highly pathogenic avian influenza H5N1 virus. Our results suggest that this recombinant protein produced in yeast may be an effective vaccine against H5N1 virus in poultry.
Cechy publikacji
praca doświadczalna
Inne
System-identifier
1155
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