Development of cross-priming amplification for direct detection of the African Swine Fever Virus, in pig and wild boar blood and sera samples
PBN-AR
Instytucja
Państwowy Instytut Weterynaryjny - Państwowy Instytut Badawczy
Informacje podstawowe
Główny język publikacji
en
Czasopismo
LETTERS IN APPLIED MICROBIOLOGY
ISSN
0266-8254
EISSN
1472-765X
Wydawca
WILEY-BLACKWELL
Rok publikacji
2016
Numer zeszytu
5
Strony od-do
386-391
Numer tomu
62
Identyfikator DOI
Liczba arkuszy
Słowa kluczowe
EN
African swine fever virus
cross-priming amplification
development
direct virus DNA detection
pigs and wild boar blood
sera
Streszczenia
Język
en
Treść
African swine fever (ASF) is considered a major threat to the production of pigs worldwide. The ASF aetiological agent, ASFV, is the sole member of the Asfivirus genus, belonging to the Asfarviridae family. An effective ASF vaccine is not currently available, thus the only measures of ASF spread control include, reliable and fast diagnosis. Officially approved, diagnostic methods include, virus isolation, serological assays, including enzyme-linked immunosorbent assay and immunoperoxidase assay (IPT) and different modifications of the polymerase chain reaction (PCR). This paper describes the first development and application of a cross-priming amplification method (CPA) for the direct detection of genetic ASFV material, in blood and sera from pigs and wild boars. This method is specific only to ASFV DNA. The study showed that CPA had equal sensitivity, in comparison to the official, universal probe library (UPL) real-time PCR and reached 7·2 copies of standard plasmid DNA, containing a p72 gene fragment. This method was capable of detecting ASFV DNA in all examined blood samples, originating from pigs; n = 10 and wild boars; n = 76. The obtained results were also confirmed by the officially approved, real-time PCR. The developed CPA might be further used by local and county veterinary officers, hunters or pig farmers, for preliminary ASF diagnosis.
Cechy publikacji
praca doświadczalna
Inne
System-identifier
934
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