Combination of therapy with 5-fluorouracil and cisplatin with electroporation in human ovarian carcinoma model in vitro
PBN-AR
Instytucja
Instytut Immunologii i Terapii Doświadczalnej im. Ludwika Hirszfelda Polskiej Akademii Nauk
Informacje podstawowe
Główny język publikacji
EN
Czasopismo
Biomedicine & pharmacotherapy
ISSN
0753-3322
EISSN
1950-6007
Wydawca
Paris : Editions Scientifiques Elsevie
Rok publikacji
2014
Numer zeszytu
5
Strony od-do
573-580
Numer tomu
68
Identyfikator DOI
Liczba arkuszy
Autorzy
(liczba autorów: 10)
Pozostali autorzy
+ 9
Słowa kluczowe
EN
Electropermeabilization
Electrochemotherapy
Ovarian carcinoma
Human fibroblasts
5-fluorouracill
Cisplatin
Streszczenia
Język
EN
Treść
High electric field, applied to plasma membrane, affects organization of the lipid molecules, generating transient hydrophilic electropores. The application of the cell membrane electroporation in combination with cytotoxic drugs could increase the drug transport into cells. This approach is known as electrochemotherapy (ECT). Our work shows new data concerning the influence of electrochemical reaction with cisplatin or with 5-fluorouracil (5-FU) on cancer ovarian cells resistant to standard therapy with cisplatin, in comparison to ECT effect on human primary fibroblasts. We investigated the effect of electroporation and electrochemotherapy with 5-FU and cisplatin on human ovarian clear-cell carcinoma cell line (OvBH-1) and epithelial ovarian carcinoma cell line (SKOV-3) - both resistant to cisplatin typically used in ovarian cancers. As control cells, human gingival fibroblasts (HGF's) from primary culture were used. Electropermeabilization efficiency was determined by FACS analysis with iodide propidium. Efficiency of electrochemotherapy was evaluated with viability assay. The cytotoxic effect was dependent on the electroporation parameters and on drug concentration. Electroporation alone only insignificantly decreased cells proliferation in OvBH-1 line; SKOV-3 line was more sensitive to the electrical field. Electrochemotherapy with cisplatin and 5-FU showed promising effects on both ovarian cell lines with recovery of normal cells revealed after 72 hours.
Cechy publikacji
artykuł oryginalny
Inne
System-identifier
PX-56b48bcb8106eb71826f81aa
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