Arginine deprivation affects glioblastoma cell adhesion, invasiveness and actin cytoskeleton organization by impairment of β-actin arginylation.
PBN-AR
Instytucja
Wydział Biologii (Uniwersytet Warszawski)
Informacje podstawowe
Główny język publikacji
en
Czasopismo
Amino Acids
ISSN
0939-4451
EISSN
1438-2199
Wydawca
SPRINGER
DOI
URL
Rok publikacji
2015
Numer zeszytu
1
Strony od-do
199-212
Numer tomu
47
Identyfikator DOI
Liczba arkuszy
Autorzy
(liczba autorów: 10)
Pozostali autorzy
+ 8
Słowa kluczowe
en
Amino acid deprivation · Actin cytoskeleton · Actin arginylation · Cancer · Invasiveness · Cell migration
Streszczenia
Język
en
Treść
A deficit of exogenous arginine affects growth and viability of numerous cancer cells. Although arginine deprivation-based strategy is currently undergoing clinical trials, molecular mechanisms of tumor cells' response to arginine deprivation are not yet elucidated. We have examined effects of arginine starvation on cell motility, adhesion and invasiveness as well as on actin cytoskeleton organization of human glioblastoma cells. We observed for the first time that arginine, but not lysine, starvation affected cell morphology, significantly inhibited their motility and invasiveness, and impaired adhesion. No effects on glia cells were observed. Also, arginine deprivation in glioblastoma evoked specific changes in actin assembly, decreased β-actin filament content, and affected its N-terminal arginylation. We suggest that alterations in organization of β-actin resulted from a decrease of its arginylation could be responsible for the observed effects of arginine deprivation on cell invasiveness and migration. Our data indicate that arginine deprivation-based treatment strategies could inhibit, at least transiently, the invasion process of highly malignant brain tumors and may have a potential for combination therapy to extend overall patient survival.
Cechy publikacji
ORIGINAL_ARTICLE
Inne
System-identifier
522463
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