Common occurrence of Gallid herpesvirus-2 (GaHV-2) with reticuloendotheliosis virus (REV) in chickens caused by possible contamination of vaccine stocks
PBN-AR
Instytucja
Państwowy Instytut Weterynaryjny - Państwowy Instytut Badawczy
Informacje podstawowe
Główny język publikacji
en
Czasopismo
JOURNAL OF APPLIED MICROBIOLOGY
ISSN
1364-5072
EISSN
1365-2672
Wydawca
WILEY-BLACKWELL
DOI
URL
Rok publikacji
2015
Numer zeszytu
4
Strony od-do
803-808
Numer tomu
118
Identyfikator DOI
Liczba arkuszy
Autorzy
Pozostali autorzy
+ 1
Słowa kluczowe
en
Gallid herpesvirus-2
chickens
co-occurrence
loop-mediated isothermal amplification
reticuloendotheliosis virus
vaccine contamination
Streszczenia
Język
en
Treść
Aims The aim of this study was to investigate the common occurrence of reticuloendotheliosis virus (REV) among Gallid herpesvirus 2 (GaHV-2) infected chickens. The possible cause of this co-occurrence may be linked to contaminated vaccine stocks, which were also examined. Methods and Results The study was conducted on 25 field isolates of GaHV-2 collected between 2007 and 2013 from vaccinated chickens. Additionally, 10 commercial Marek's Disease vaccine stocks manufactured between 1993 and 2013, comprising of FC126 HVT, CVI988/Rispens and bivalent HVT + Rispens vaccines were examined. Chicken isolates were collected from the liver. Due to difficulties in differentiation between GaHV-2 and REV, by observation of clinical signs or lesions presented in liver or spleen, loop-mediated isothermal amplification (LAMP and RT-LAMP) as well as PCR-based methods were applied. Conclusions The co-occurrence of GaHV-2 and REV genetic material was shown in 24 (96%) of 25 examined isolates. A marginal REV contamination was detected in three out 10 (30%) commercial vaccine stocks, mainly in bivalent HVT + Rispens vaccines produced between 2009 and 2012. Significance and Impact of the Study Our results indicated the common occurrence of GaHV-2 and REV in Polish chicken flocks, which is probably linked to contaminated HVT + Rispens vaccine stocks. Reasons for the detection of a marginal REV contamination need to be further elucidated.
Cechy publikacji
ORIGINAL_ARTICLE
Inne
System-identifier
597497
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