Prevalence and Characterization of Quinolone Resistance Mechanisms in Commensal Escherichia coli Isolated from Slaughter Animals in Poland, 2009-2012
PBN-AR
Instytucja
Państwowy Instytut Weterynaryjny - Państwowy Instytut Badawczy
Informacje podstawowe
Główny język publikacji
en
Czasopismo
Microbial Drug Resistance
ISSN
1076-6294
EISSN
1931-8448
Wydawca
MARY ANN LIEBERT INC
DOI
URL
Rok publikacji
2014
Numer zeszytu
6
Strony od-do
544-549
Numer tomu
20
Identyfikator DOI
Liczba arkuszy
Autorzy
(liczba autorów: 1)
Słowa kluczowe
en
quinolone resistance mechanisms
commensal Escherichia coli
slaughter animals
Poland
2009-2012
Streszczenia
Język
en
Treść
The background of quinolone resistance was characterized in ciprofloxacin-resistant commensal Escherichia coli selected out of 3,551 isolates from slaughtered animals in Poland between 2009 and 2012. Plasmid-mediated determinants were suspected in 6.2% of the study group, ranging from 1.1% in cattle to 9.7% in turkeys. Polymerase chain reaction and sequencing identified up to four quinolone resistance-determining substitutions in gyrA (Ser83, Asp87) and parC (Ala56, Ser80). Plasmid-mediated mechanisms were identified as qnrS1 (or qnrS3, n=70, including six isolates with chromosomal mutations), qnrB19 (or qnrB10, n=19), and qnrB17 (n=1). All tested isolates were negative for qnrA, qnrC, qnrD, qepA, and aac(6′)-Ib-cr. Still, there were several E. coli suspected for both plasmid- and chromosome-mediated resistance with unrevealed genetic background of the phenomenon. Since all tested isolates showed diverse XbaI-PFGE profiles, chromosome-encoded quinolone resistance does not result from the spread of a single resistant clone, however, it is rather due to antimicrobial pressure leading to the selection of random gyr and par mutants. It also favors the selection and spread of plasmids carrying predominant qnr genes, since the same determinants were found in Salmonella, isolated from similar sources. The identification of carrier plasmids and mitigation of their spread might be essential for sustainable quinolone usage in animal husbandry and efficient protection of human health.
Cechy publikacji
praca doświadczalna
Inne
System-identifier
553866
CrossrefMetadata from Crossref logo
Cytowania
Liczba prac cytujących tę pracę
Brak danych
Referencje
Liczba prac cytowanych przez tę pracę
Brak danych