Detection of CoHV-1 by loop-mediated amplification (LAMP). Application of LAMP for CoHV-1 incidence monitoring in domestic pigeons
PBN-AR
Instytucja
Państwowy Instytut Weterynaryjny - Państwowy Instytut Badawczy
Informacje podstawowe
Główny język publikacji
en
Czasopismo
LETTERS IN APPLIED MICROBIOLOGY
ISSN
0266-8254
EISSN
1472-765X
Wydawca
WILEY-BLACKWELL
DOI
URL
Rok publikacji
2014
Numer zeszytu
6
Strony od-do
610-614
Numer tomu
59
Identyfikator DOI
Liczba arkuszy
Autorzy
Pozostali autorzy
+ 1
Słowa kluczowe
en
Columbid herpesvirus-1
Pigeons
Smadel disease
loop-mediated isothermal amplification
Streszczenia
Język
en
Treść
The occurrence of Columbid herpesvirus-1 (CoHV-1) in domestic pigeons presents a potential threat for their husbandry and trade. CoHV-1 causes Smadel disease among pigeons but may not be manifested by any clinical signs and complicates secondary infections. The material for our study originated from 42 domestic pigeons sent to private veterinary practice in Lublin, Poland, between 2011 and 2013. Some of birds showed clinical signs similar to Smadel disease. The cytological examination also indicated on CoHV-1 infection. The incidence of CoHV-1 was tested in DNA extracted from liver of birds by loop-mediated isothermal amplification (LAMP). LAMP was used for the monitoring of CoHV-1 presence among pigeons in Poland. Our study showed that LAMP was capable of detecting CoHV-1 presence in 8 (19%) of 42 examined birds without the use of any advanced laboratory equipment. The results were confirmed by real-time PCR and virus isolation in chicken embryo fibroblasts. This is the first report on LAMP application for successful detection of CoHV-1 in domestic pigeons.
Cechy publikacji
praca doświadczalna
Inne
System-identifier
553889
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