Proangiogenic Properties of Nucleoside 5'-O-Phosphorothioate Analogues Under Hyperglycaemic Conditions
PBN-AR
Instytucja
Wydział Biotechnologii i Nauk o Żywności (Politechnika Łódzka)
Informacje podstawowe
Główny język publikacji
en
Czasopismo
CURRENT TOPICS IN MEDICINAL CHEMISTRY
ISSN
1568-0266
EISSN
Wydawca
BENTHAM SCIENCE PUBL LTD
DOI
URL
Rok publikacji
2015
Numer zeszytu
23
Strony od-do
2464-2474
Numer tomu
15
Link do pełnego tekstu
Liczba arkuszy
Słowa kluczowe
en
Angiogenesis
Hyperglycaemia
Keratinocytes
Nucleoside-5'-O-phosphorothioate analogues
P2Y receptors
VEGF
Wound healing
Streszczenia
Język
en
Treść
Diabetes leads to impairment of the normal course of wound healing. Interestingly, recent studies have implicated a critical role of P2X/P2Y nucleotide receptors in dermal tissue regeneration and maintaining vascular homeostasis. As new vessel generation and keratinization process are decreased in diabetic patients we determined whether nucleoside 5'-O-phosphorothioate analogues might accelerate vascular endothelial growth factor (VEGF) production as well as the growth and migration of human keratinocytes under hyperglycaemic conditions. We also investigated the expression pattern of P2X/P2Y receptors in human keratinocyte HaCaT cells. We show here that nucleoside 5'-O-phosphorothioate analogues are better candidates to overcome hyperglycaemia-induced impairment of angiogenesis as compared to their unmodified counterparts. The greatest potency for VEGF release and stimulation of cell migration by thiophosphate analogues of ATP and UTP correlates with the highest P2Y2 receptor expression by HaCaT cells. We also found that UTPαS significantly increased the viability and proliferation of the HaCaT cells. These findings suggest that thiophosphate analogues of nucleotides could serve as potential therapeutic agents for promoting impaired angiogenesis under diabetic conditions.
Cechy publikacji
ORIGINAL_ARTICLE
Inne
System-identifier
576767
CrossrefMetadata from Crossref logo
Cytowania
Liczba prac cytujących tę pracę
Brak danych
Referencje
Liczba prac cytowanych przez tę pracę
Brak danych