Exercise-induced heart mitochondrial cholesterol depletion influences the inhibition of mitochondrial swelling
PBN-AR
Instytucja
Wydział Psychologii (SWPS Uniwersytet Humanistycznospołeczny z siedzibą w Warszawie)
Informacje podstawowe
Główny język publikacji
en
Czasopismo
Experimental Physiology
ISSN
0958-0670
EISSN
Wydawca
DOI
URL
Rok publikacji
2013
Numer zeszytu
10
Strony od-do
1457-1468
Numer tomu
98
Identyfikator DOI
Liczba arkuszy
0.55
Streszczenia
Język
en
Treść
The significance of the reduction of the cholesterol pool in heart mitochondria after exercise is still unknown. Recently, published data have suggested that cholesterol may influence the components of mitochondrial contact site and affectmitochondrial swelling. Therefore, the aim of this study was to determine whether the decreased cholesterol content in heartmitochondria caused by prolonged swimming may provoke changes in their bioenergetics and result in an increased resistance to calcium chloride-induced mitochondrial swelling.MaleWistar rats were divided into a sedentary control group and an exercise group. The rats exercised for 3 h, burdened with an additional 3% of their body weight. Their hearts were removed immediately after completing the exercise. The left ventricle was divided and used for experiments.Mitochondrial cholesterol content, membrane fluidity and mitochondrial bioenergetics were measured in the control and exercised rat heart mitochondria. To assess whether mitochondrial modifications are linked to disruption of lipid microdomains, methyl-β-cyclodextrin, a well-known lipid microdomain-disrupting agent and cholesterol chelator, was applied to the mitochondria of the control group. Cholesterol depletion, increased membrane fluidity and increased resistance to calcium chloride-induced swelling were observed in postexercise heart crude mitochondrial fraction. Similar results were achieved in control mitochondria treated with 2% methyl-β- cyclodextrin.All of themitochondrial bioenergetics parameterswere similar between the groups.
Inne
System-identifier
IMPORT-ARTICLE-00013829
CrossrefMetadata from Crossref logo
Cytowania
Liczba prac cytujących tę pracę
Brak danych
Referencje
Liczba prac cytowanych przez tę pracę
Brak danych