The importance of TRPV1-sensitisation factors for the development of neuropathic pain
PBN-AR
Instytucja
Instytut Farmakologii im. Jerzego Maja Polskiej Akademii Nauk
Źródłowe zdarzenia ewaluacyjne
Informacje podstawowe
Główny język publikacji
angielski
Czasopismo
MOLECULAR AND CELLULAR NEUROSCIENCE
ISSN
1044-7431
EISSN
Wydawca
ELSEVIER
DOI
Rok publikacji
2015
Numer zeszytu
Strony od-do
1-10
Numer tomu
65
Identyfikator DOI
Liczba arkuszy
Słowa kluczowe
en
TRPV1 sensitisation
DRG
Neuropathic pain
Neuronal inflammation
CCI
Streszczenia
Język
en
Treść
Transient receptor potential vanilloid type 1 (TRPV1), classically associated with transduction of high-temperature and low-pH pain, underlies pain hypersensitivity in neuropathic pain. The molecular regulation of TRPV1 channel activity is not yet fully understood. Therefore, we investigated factors regulating sensitisation of this receptor during development of neuropathic pain in a rat model of chronic construction injury (CCI) in the dorsal root ganglia (DRG). In the rat CCI model, elevated levels of pro-inflammatory cytokines (TNF alpha, IL-1 beta and IL-6) in DRG corresponded to development of neuropathic pain. We assessed the expression of known kinases influencing TRPV1 sensitisation at the mRNA and/or protein level. Protein kinase C epsilon (PKC epsilon) showed the strongest upregulation at the mRNA and protein levels among all tested kinases. Co-expression of PKC epsilon and TRPV1 in L5 DRG of CCI animals was high during the development of neuropathic pain. The number of neurons expressing PKCs increased throughout the experiment We provide complex data on the expression of a variety of factors involved in TRPV1 sensitisation in a CCI model of neuropathic pain. Our study supports evidence for involvement of TRPV1 in the development of neuropathic pain, by showing increased expression of interleukins and kinases responsible for the channel sensitisation. TNF alpha and NGF seem to play a role in the transition from acute to neuropathic pain, while PKCs in its maintenance. Further studies might confirm their significance as novel targets for the treatment of neuropathic pain. (C) 2015 Elsevier Inc. All rights reserved.
Cechy publikacji
ORIGINAL_ARTICLE
Inne
System-identifier
660217
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