Contact pin-printing of albumin-fungicide conjugate for silicon nitride-based sensors biofunctionalization: multi-technique surface analysis for optimum immunoassay performance
PBN-AR
Instytucja
Wydział Fizyki i Informatyki Stosowanej (Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie)
Źródłowe zdarzenia ewaluacyjne
Informacje podstawowe
Główny język publikacji
EN
Czasopismo
Applied Surface Science (35pkt w roku publikacji)
ISSN
0169-4332
EISSN
1873-5584
Wydawca
Elsevier Science BV
DOI
Rok publikacji
2017
Numer zeszytu
Strony od-do
79--86
Numer tomu
410
Link do pełnego tekstu
Identyfikator DOI
Liczba arkuszy
0.57
Słowa kluczowe
EN
atomic force microscopy
time-of-flight secondary ion mass spectrometry
silicon nitride
spectroscopic ellipsometry
contact pin-printing
immunosensor biofunctionalization
Streszczenia
Język
EN
Treść
Mass fabrication of integrated biosensors on silicon chips is facilitated by contact pin-printing, applied for biofunctionalization of individual Si3N4-based transducers at wafer-scale. To optimize the biofunctionalization for immunochemical (competitive) detection of fungicide thiabendazole (TBZ), Si3N4surfaces are modified with (3-aminopropyl)triethoxysilane and examined after: immobilization of BSA-TBZ conjugate (probe) from solutions with different concentration, blocking with bovine serum albumin (BSA), and immunoreaction with a mouse monoclonal antibody against TBZ. Nanostructure, surface density, probe composition and coverage uniformity of protein layers are evaluated with Atomic Force Microscopy, Spectroscopic Ellipsometry, Time-of-Flight Secondary Ion Mass Spectrometry and X-ray Photoelectron Spectroscopy. Contact pin-printing of overlapping probe spots is compared with hand spotted areas. Contact pin-printing resulted in two-fold increase of immobilized probe surface density as compared to hand spotting. Regarding BSA-TBZ immobilization, an incomplete monolayer develops into a bilayer as the concentration of BSA-TBZ molecules in the printing solution increases from 25 to 100 μg/mL. Upon blocking, however, a complete protein monolayer is formed for all the BSA-TBZ concentrations used. Free surface sites are filled with BSA for low surface coverage with BSA-TBZ, whereas loosely bound BSA-TBZ molecules are removed from the BSA-TBZ bilayer. As a consequence immunoreaction efficiency increases with the printing probe concentration. © 2017 Elsevier B.V.
Cechy publikacji
original article
peer-reviewed
Inne
System-identifier
idp:105529
CrossrefMetadata from Crossref logo
Cytowania
Liczba prac cytujących tę pracę
Brak danych
Referencje
Liczba prac cytowanych przez tę pracę
Brak danych