Imaging and chemical surface analysis of biomolecular functionalization of monolithically integrated on silicon Mach-Zehnder interferometric immunosensors
PBN-AR
Instytucja
Akademickie Centrum Materiałów i Nanotechnologii (Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie)
Źródłowe zdarzenia ewaluacyjne
Informacje podstawowe
Główny język publikacji
EN
Czasopismo
Applied Surface Science
ISSN
0169-4332
EISSN
1873-5584
Wydawca
Elsevier Science BV
Rok publikacji
2016
Numer zeszytu
Strony od-do
529--542
Numer tomu
385
Link do pełnego tekstu
Identyfikator DOI
Liczba arkuszy
1
Autorzy
Pozostali autorzy
+ 5
Słowa kluczowe
EN
atomic force microscopy
time-of-flight secondary ion mass spectrometry
X-ray photoelectron spectroscopy
multi-molecular coverage
on-chip immunoassay
biosensor functionalization
Streszczenia
Język
EN
Treść
Time-of-flight secondary ion mass spectrometry (imaging, micro-analysis) has been employed to evaluate biofunctionalization of the sensing arm areas of Mach-Zehnder interferometers monolithically integrated on silicon chips for the immunochemical (competitive) detection of bovine kappa-casein in goat milk. Biosensor surfaces are examined after: modification with (3-aminopropyl)triethoxysilane, application of multiple overlapping spots of kappa-casein solutions, blocking with 100-times diluted goat milk, and reaction with monoclonal mouse anti-kappa-casein antibodies in blocking solution. The areas spotted with kappa-casein solutions of different concentrations are examined and optimum concentration providing homogeneous coverage is determined. Coverage of biosensor surfaces with biomolecules after each of the sequential steps employed in immunodetection is also evaluated with TOF-SIMS, supplemented by Atomic force microscopy and X-ray photoelectron spectroscopy. Uniform molecular distributions are observed on the sensing arm areas after spotting with optimum kappa-casein concentration, blocking and immunoreaction. The corresponding biomolecular compositions are determined with a Principal Component Analysis that distinguished between protein amino acids and milk glycerides, as well as between amino acids characteristic for Mabs and kappa-casein, respectively. Use of the optimum conditions (kappa-casein concentration) for functionalization of chips with arrays of ten Mach-Zehnder interferometers provided on-chips assays with dramatically improved both intra-chip response repeatability and assay detection sensitivity. (C) 2016 Elsevier B.V. All rights reserved.
Cechy publikacji
original article
peer-reviewed
Inne
System-identifier
idp:101988
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