Dark-induced senescence of barley leaves involves activation of plastid transglutaminases
PBN-AR
Instytucja
Wydział Ogrodnictwa i Architektury Krajobrazu (Uniwersytet Przyrodniczy w Poznaniu)
Źródłowe zdarzenia ewaluacyjne
Informacje podstawowe
Główny język publikacji
angielski
Czasopismo
AMINO ACIDS
ISSN
0939-4451
EISSN
1438-2199
Wydawca
DOI
URL
Rok publikacji
2015
Numer zeszytu
4
Strony od-do
825-838
Numer tomu
47
Identyfikator DOI
Liczba arkuszy
Słowa kluczowe
angielski
chloroplast
leaf
Hv-Png1-like gene
polyamines
senescence
transglutaminase
Streszczenia
Język
angielski
Treść
Transglutaminases (E.C. 2.3.2.13) catalyze the post-translational modification of proteins by establishing ε-(γ-glutamyl) lysine isopeptide bonds and by the covalent conjugation of polyamines to endo-glutamyl residues of proteins. In light of the confirmed role of transglutaminases in animal cell apoptosis and only limited information on the role of these enzymes in plant senescence, we decided to investigate the activity of chloroplast transglutaminases (ChlTGases) and the fate of chloroplast-associated polyamines in Hordeum vulgare L. 'Nagrad' leaves, where the senescence process was induced by darkness (day 0) and continued until chloroplast degradation (day 12). Using an anti-TGase antibody, we detected on a subcellular level, the ChlTGases that were associated with destacked/degraded thylakoid membranes, and beginning on day 5, were also found in the stroma. Colorimetric and radiometric assays revealed during senescence an increase in ChlTGases enzymatic activity. The MS/MS identification of plastid proteins conjugated with exogenous polyamines had shown that the ChlTGases are engaged in the post-translational modification of proteins involved in photosystem organization, stress response, and oxidation processes. We also computationally identified the cDNA of Hv-Png1-like, a barley homologue of the Arabidopsis AtPng1 gene. Its mRNA level was raised from days 3 to 10, indicating that transcriptional regulation controls the activity of barley ChlTGases. Together, the presented results deepen our knowledge of the mechanisms of the events happened in dark-induced senescence of barley leaves that might be activation of plastid transglutaminases.
Cechy publikacji
original-article
Inne
System-identifier
0000012065
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