The influence of very small doses of alpha radiation on the stability of erythrocytes
PBN-AR
Instytucja
Wydział Fizyki i Informatyki Stosowanej (Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie)
Źródłowe zdarzenia ewaluacyjne
Informacje podstawowe
Główny język publikacji
EN
Czasopismo
Microscopy Research and Technique (25pkt w roku publikacji)
ISSN
1059-910X
EISSN
1097-0029
Wydawca
Wiley-Blackwell
DOI
Rok publikacji
2017
Numer zeszytu
1
Strony od-do
131--143
Numer tomu
80
Link do pełnego tekstu
Identyfikator DOI
Liczba arkuszy
0.92
Słowa kluczowe
EN
atomic force microscopy
Mössbauer spectroscopy
red blood cell
membrane skeleton
α-radiation
Streszczenia
Język
EN
Treść
Our aim was to study the influence of low doses (0.2-4 mu Gy) of alpha radiation on the stability of human erythrocytes isolated from healthy and diabetic erythrocytes. Absorption spectroscopy was used to measure the level of red blood cell (RBC) hemolysis, along with Mossbauer spectroscopy, which is a highly specific method suited to monitoring various hemoglobin forms. States of hemoglobin are sensitive to a homeostatic imbalance in red blood cells. Changes in the membrane skeleton organization of irradiated erythrocytes isolated from healthy donors were studied using atomic force microscopy (AFM). Hemolysis, in healthy red blood cells, showed characteristic discontinuities, depending on the a particle flux and the exposure time to the low doses applied. This phenomenon was not observed in severe diabetic cases, which could be a result of modified protein-lipid-sugar complexes and the attenuation/absence of some antioxidative enzymatic processes in their RBC membranes. Similar effects were also observed for red blood cells treated with low doses of neutron and gamma-radiation. AFM measurements demonstrated a reorganization of the RBC membrane skeleton network depending on the time of RBC exposure to alpha radiation. This suggests that the changes in the activity of the acute defense processes against free radicals which are activated within the erythrocyte membrane irradiated with a-particles could additionally be up-or down regulated by modifications to the membrane-skeleton network. However, even the highest dose of alpha radiation applied in these studies did not cause any significant changes in the ability of hemoglobin to transport oxygen.
Cechy publikacji
original article
peer-reviewed
Inne
System-identifier
idp:104543
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